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reference · compounds

Beta-glucan content reference

Beta-glucans are the primary bioactive compounds in functional mushrooms responsible for immune modulation, and their co...

SpeciesFormBeta-glucan %NoteSource
Lion's ManeDual extract25–40%Quality benchmark ≥25%PubMed 28244676
Lion's ManeHot water20–35%Lower concentration than dual
ReishiDual extract25–35%Also contains triterpenesPubMed 22207209
ReishiHot water20–30%Triterpenes reduced
Turkey TailHot water30–45%PSK fraction key markerPubMed 22203167
CordycepsDual extract15–25%CS-4 strain studied clinicallyPubMed 22700032
ChagaHot water20–30%Also contains betulinic acidPubMed 21820502
MaitakeHot water20–35%D-fraction isolated markerPubMed 23668749
ShiitakeHot water25–40%AHCC proprietary fractionPubMed 25866155
AgaricusHot water15–30%Beta-1,3/1,6-D-glucanPubMed 11413477

For informational and research purposes only. Not intended to diagnose, treat, cure, or prevent any disease. Consult a qualified healthcare provider before beginning any supplement protocol. Individual responses to supplementation vary.

About this calculator

Beta-glucans are the primary bioactive compounds in functional mushrooms responsible for immune modulation, and their concentration in a supplement is the single most reliable quality indicator. This reference table compiles beta-glucan percentage ranges from published extraction studies and third-party testing standards used by leading wholesale suppliers. The 25% threshold is widely used as a minimum quality benchmark for standardised functional mushroom extracts — products below this threshold are likely mycelium-on-grain products with high starch content rather than true fruiting body extracts. Use this table for: comparing products when purchasing, verifying COA claims, understanding which species and extraction methods produce the highest active compound concentrations, and planning stacks based on beta-glucan density.

Frequently asked

What is a good beta-glucan percentage in a mushroom supplement?

25% or higher in a standardised dual extract is the widely accepted quality benchmark. Products below 10% are likely mycelium on grain with significant starch filler rather than true fruiting body extract.

How is beta-glucan content measured?

Beta-glucan content is measured using enzymatic assay methods (the Megazyme method is the gold standard). Results should be expressed as % beta-glucan per dry weight and verified by an independent third-party lab.

Why does turkey tail have the highest beta-glucan content?

Trametes versicolor has evolved highly concentrated polysaccharide fractions (PSK and PSP) as part of its immune-defence function. These same compounds are responsible for its powerful human immune effects.

Do all mushroom species have the same beta-glucans?

No — the structural configuration of beta-glucans varies between species. Turkey tail has beta-1,3/1,6-D-glucan (PSK). Lion's mane has unique hericenone-adjacent polysaccharides. Shiitake has lentinan (beta-1,3-glucan). Each interacts with immune receptors differently.

What is the difference between beta-glucan and alpha-glucan?

Beta-glucans are the bioactive immune compounds in functional mushrooms. Alpha-glucans are starches — they are inert from an immune perspective. Mycelium-on-grain products have high alpha-glucan (starch) content from the grain substrate, which inflates total polysaccharide numbers without providing beta-glucan activity.

Research background

Beta-glucan quantification in functional mushrooms uses the Megazyme assay, which specifically measures the (1,3)/(1,4)-beta-D-glucan fraction. The ranges in this table are based on extraction studies and COA benchmarks from Nammex (North American Medicinal Mushroom Extracts), the industry-standard organic wholesale supplier, cross-referenced with published analytical chemistry papers.

For informational purposes only. Not intended to diagnose, treat, cure, or prevent any disease. Consult a qualified healthcare provider before beginning any supplement protocol.